A Therapeutic Approach for Wound Healing by Using Essential Oils of Cupressus and Juniperus Species Growing in Turkey

Figure 1

Histopathological view of wound healing and epidermal/dermal remodeling in the vehicle, negative control, oils, and reference ointment Madecassol-administered animals. Skin sections show the hematoxylin & eosin- (HE-) stained epidermis and dermis in A and the dermis stained with Van Gieson (VG) in B. The original magnification was ×100 and the scale bars represent 120 μm for figures in A, and the original magnification was ×400 and the scale bars represent 40 μm for B. Data are representative of 6 animals per group. (1) Vehicle group: 10-day-old wound tissue treated with only vehicle, (2) negative control group: 10-day-old wound tissue, untreated group, (3) C. sempervirens var. horizontalis group: 10-day-old wound tissue treated with the essential oil of C. sempervirens var. horizontalis, (4) C. sempervirens var. pyramidalis group, 10 day old wound tissue treated with essential oil of C. sempervirens var. pyramidalis, (5) J. communis group: 10-day-old wound tissue treated with essential oil of J. communis, (6) J. excelsa group: 10-day-old wound tissue treated with essential oil of J. excelsa, (7) J. foetidissima group: 10-day-old wound tissue treated with essential oil of J. foetidissima, (8) J. oxycedrus group: 10-day-old wound tissue treated with essential oil of J. oxycedrus, (9) J. phoenicea group: 10-day-old wound tissue treated with essential oil of J. phoenicea, and (10) reference group: 10-day-old wound tissue treated with Madecassol. Arrows pointing events during wound healing; s: scab, u: ulcer, re: re-epithelization, f: fibroblast, c: collagen, mnc: mononuclear cells, pmn: polymorphonuclear cells, and nv: neovascularization.