Effect of GCSB-5, a Herbal Formulation, on Monosodium Iodoacetate-Induced Osteoarthritis in Rats

Figure 1

GAG release in rabbit articular cartilage explant cultures at 24, 48, and 72 h. Rabbit articular cartilage explants were stimulated with rhIL-1α (5 ng/mL). The amount of GAG release stimulated by rhIL-1α (∘) increased approximately 3.6 times compared to control (●) at 72 h. GCSB-5 (1.0×10⁻³ (▼) and 1.0×10⁻² (∆) mg/mL) and diclofenac (30 μM (□)) efficiently inhibited the GAG release. However, a high concentration of GCSB-5 (1.0×10⁻¹ (■) mg/mL) slightly inhibited it. Each value represents the mean ± S.E.M. from 6 articular cartilage explants cultures per group. **Significantly different (𝑃<0.01) from control. ⁺ and ⁺⁺Significantly different (𝑃<0.05, 𝑃<0.01) from rhIL-1α.