Antileukemic Potential of Momordica charantia Seed Extracts on Human Myeloid Leukemic HL60 Cells
Figure 2
(a) Effect of acetone extract of Mc-2 (Mc-2Ac) on HL60 cells differentiation. Mc-2Ac-treated (5–20 μg/mL) cells showed a significant increase in NBT positive cells on day 4 and day 5, respectively, compared to untreated cells. (b) Time-dependence of Mc-2Ac-induced HL60 cells differentiation. Mc-2Ac-treated HL60 cells were scored for NBT-positive cells from day 1 to 5. DMSO was included as a positive control. (c) Effect of fraction Mc-2Ac on HL60 cells viability. HL60 cells (105/mL) cultured in a 96-well plate were treated with the indicated concentrations of fraction Mc-2Ac for 72 h. Cell viability was assessed by MTT assay. The optical density values of untreated control cells were taken as 100%. The data represent the mean + SD of three independent experiments. (d) Minimum time required for Mc-2Ac to induce HL60 differentiation. HL60 cells cultured in the presence of Mc-2Ac (20 μg/mL) for the indicated time, followed by growth in the medium without Mc-2Ac, were scored for NBT-positive cells on day 5. Data in all the panels represent the mean ± S.D of 3 independent experiments performed in triplicates. *, **, and *** relative to untreated cells.
