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Evidence-Based Complementary and Alternative Medicine
Volume 2012, Article ID 732404, 10 pages
http://dx.doi.org/10.1155/2012/732404
Research Article

Antileukemic Potential of Momordica charantia Seed Extracts on Human Myeloid Leukemic HL60 Cells

1Gene Regulation Laboratory, School of Biotechnology, Jawaharlal Nehru University, New Delhi 110 067, India
2Department of Zoology, University of Delhi, New Delhi 110 007, India

Received 7 January 2012; Revised 20 February 2012; Accepted 21 February 2012

Academic Editor: Raffaele Capasso

Copyright © 2012 Ramani Soundararajan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Table 1A: Effect of different fractions of M. charantia seeds on HL60 cells differentiation on day 5 of the treatment. HL60 cells treated with varying concentration (5-20 µg/ml) of different fractions (Mc-1, Mc-2 and Mc-3) were scored for NBT positive cells on day 5. The data represent total number of cells, and total number of NBT positive cells in the respective samples scored in three wells (triplicates) for each experimental conditions.

Supplementary Table 1B: Time dependence of Mc-2 induced HL60 cells differentiation. HL60 cells were treated with Mc-2 (20 µg/ml) and DMSO (1%) and scored for NBT staining from day 1 to 5. DMSO was included as a positive control. The data represent total number of cells, and total number of NBT positive cells in the respective samples scored in three wells (triplicates) for each experimental conditions.

Supplementary Table 1C: Effect of heat-inactivated Mc-2 (Mc-2H) and protease-treated Mc-2 (Mc-2P) on HL60 differentiation. HL60 cells treated with Mc-2H and Mc-2P (20 µg/ml each) were scored for NBT positive cells. The data represent total number of cells, and total number of NBT positive cells in the respective samples scored in three wells (triplicates) for each experimental conditions.

Supplementary Table 1D: Effect of acetone extract of Mc-2 (Mc-2Ac) on HL60 differentiation. Mc-2Ac - treated (5-20 µg/ml) were scored for NBT positive cells on day 4 and day 5, respectively. The data represent total number of cells, and total number of NBT positive cells in the respective samples scored in three wells (triplicates) for each experimental conditions.

Supplementary Table 1E: Time dependence of Mc-2Ac induced HL60 differentiation. Mc-2Ac-treated HL60 cells were scored for NBT-positive cells from day 1 to day 5. DMSO was included as a positive control. The data represent total number of cells, and total number of NBT positive cells in the respective samples scored in three wells (triplicates) for each experimental conditions.

Supplementary Table 1F: Minimum time required for Mc-2Ac to induce differentiation. HL60 cells cultured in the presence of Mc-2Ac for the indicated time, followed by growth in medium without Mc-2Ac were scored for NBT-positive cells on day 5. The data represent total number of cells, and total number of NBT positive cells in the respective samples scored in three wells (triplicates) for each experimental conditions.

  1. Supplementary Material