Caffeic Acid Phenethyl Ester Inhibits Oral Cancer Cell Metastasis by Regulating Matrix Metalloproteinase-2 and the Mitogen-Activated Protein Kinase Pathway
Figure 7
Effects of CAPE on the MAPKs pathway and Akt signalings. SCC-9 cells were cultured in various concentrations of CAPE (0, 5, 10, 20, and 40 μM) for 24 hours, and then the cell lysates were subjected to SDS-PAGE followed by western blots with (a) anti-ERK1/2, (b) anti-p38, (c) anti-JNK, and (d) anti-Akt (total and phosphorylated) antibodies as described in Section 2. Determined activities of these proteins were subsequently quantified by densitometric analyses with that of control being 100% as shown just after the gel data. The values represented the means ± SD of at least 3 independent experiments. * as compared with the vehicle group.
