Research Article

Explore the Molecular Mechanism of Apoptosis Induced by Tanshinone IIA on Activated Rat Hepatic Stellate Cells

Figure 5

(a) Western blot analysis for phosphorylation and total protein levels of ERK1/2 and AKT with or without PD98059 for 0.5 h. Then the cells were treated with or without Tan IIA for 6 h. The phosphorylation levels of ERK1/2 and AKT were normalized by total protein levels. Results represent the mean ± SD of three independent experiments (* ). (b) The protein levels of Bax, Bcl-2, and cytochrome c and (c) cleavage of caspase-3, caspase-9, and PARP with or without treatments of PD98059 and Tan IIA were determined by western blotting assays. Density ratio of Bax over Bcl-2 was measured by densitometer, and GAPDH was used as an internal control. The quantified results were indicated by the bar chart.