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Evidence-Based Complementary and Alternative Medicine
Volume 2012, Article ID 736752, 9 pages
http://dx.doi.org/10.1155/2012/736752
Research Article

Beneficial Effects of Ocimum gratissimum Aqueous Extract on Rats with CCl4-Induced Acute Liver Injury

1Institute of Microbiology and Immunology, Chung Shan Medical University, Taichung 40201, Taiwan
2Department of Neurology and Department of Medical Intensive Care Unit, Chunghua Christian Hospital, Chunghua 500, Taiwan
3Graduate Institute of Chinese Medical Science, China Medical University, Taichung 40402, Taiwan
4Institute of Basic Medical Science, China Medical University, Taichung 40402, Taiwan
5Department of Health and Nutrition Biotechnology, Asia University, Taichung 41354, Taiwan
6Department of Internal Medicine, Chung Shan Medical University Hospital, Taichung 40201, Taiwan
7Institute of Medicine, Chung Shan Medical University, Taichung 40201, Taiwan
8Institute of Biochemistry and Biotechnology, Chung Shan Medical University, Taichung 40201, Taiwan
9Clinical Laboratory, Chung Shan Medical University Hospital, Taichung 40201, Taiwan
10Center for Molecular Medicine, China Medical University Hospital, Taichung 40402, Taiwan
11Department of Biochemistry, School of Medicine, Chung Shan Medical University, Taichung 40201, Taiwan

Received 9 March 2012; Revised 9 April 2012; Accepted 24 April 2012

Academic Editor: Hong Zhang

Copyright © 2012 Chun-Ching Chiu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Ocimum gratissimum (OG) is known as a food spice and traditional herb, which has been recommended for the treatment of various diseases. To investigate the hepatoprotective effect of OG aqueous extract (OGAE), male Wistar rats challenged by carbon tetrachloride (CCl4) were used as the animal model of chronic hepatic injury. Significantly increased serum catalase and DPPH levels were detected in CCl4-administrated rats that were treated with OGAE or silymarin as compared to those rats that were treated with saline or CCl4. In contrast, significantly decreased stress proteins including HSP70 and iNOS were observed in livers of CCl4-administrated rats that were treated with OGAE or sylimarin as compared to those rats that were treated with saline or CCl4. Moreover, significant decreases of MMP-9/MMP-2 ratio, uPA, phosphorylated ERK (p-ERK) and NF-κB (p-P65) were detected in livers of CCl4-administrated rats that were treated with OGAE or sylimarin as compared to those rats that were treated with saline or CCl4. These findings imply that OGAE can efficiently inhibit CCl4-induced liver injuries in rats and may therefore be a potential food or herb for preventing liver injuries.