Angelicae Dahuricae Radix Inhibits Dust Mite Extract-Induced Atopic Dermatitis-Like Skin Lesions in NC/Nga Mice

Figure 3

Inhibitory effects on NO and TARC by Angelicae Dahuricae Radix (AR). The concentrations of NO and TARC in the supernatant were determined using NO and TARC ELISA assays. (a) RAW 264.7 cells were treated with LPS (1 μg/mL) for 18 hr. AR inhibited NO production in a concentration-dependent manner (mean ± SEM (𝑛=5), ^#: 𝑃<0.05, ^##: 𝑃<0.01 compared with control group, *: 𝑃<0.05, **: 𝑃<0.01 compared with the LPS-treated group). (b) HaCaT cells were treated with TNF-α plus IFN-γ (TI) (10 ng/mL each) for 24 h. TARC levels were significantly suppressed by AR (mean ± SEM, 𝑛=5), ^#: 𝑃<0.05, ^##: 𝑃<0.01 compared with control group, *: 𝑃<0.05, **: 𝑃<0.01 compared with TI treatment. (c) The MDC and TARC mRNA expression levels were determined by RT-PCR analysis, and AR suppressed the mRNA expression of MDC and TARC. The cells were treated with silymarin (10, 20, or 50 μg/mL), forskolin (5, 10, or 30 μg/mL), or AR (60, 150, or 300 μg/mL).

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