Flavonoid Myricetin Modulates
Receptor Activity through Activation of Channels and CaMK-II Pathway
Myricetin activates L-type and T-type Ca2+ channels in PVN neurons. (a) Representative recording showing Ca2+ current changes in the absence and presence of myricetin (5 μg/mL) in a typical neuron. (b) The statistical data (t-test) demonstrate a significant increase of peak current after treatment with myricetin (, *). (c) Effects of myricetin on the Ca2+ channel properties in PVN neurons. The current was recorded in response to a 500 ms ramp pulse depolarization from a holding potential of −80 mV to +60 mV, the peak current was increased and shifted to hyperpolarizing direction after myricetin treatment. (d) Summarized current-voltage relationship curves showed the currents amplitude was increased in the region between −30 mV and −10 mV; the peak currents were shifted about 10 mV to negative direction (, *, **). (e) Effects of myricetin on the different types of Ca2+ currents in PVN neurons. Representative recording showing the currents changes in the presence (red color) and absence (black color) of myricetin. Myricetin enhanced both T-type and L-type Ca2+ currents. The currents were recorded in response to a pulse shown in this figure. The first part of the current can be blocked by 300 μM NiCl2 () and the second part can be blocked by 30 μM nifedipine () (blue color). (f) The pooled data summarize the effect of myricetin on Ca2+ currents when depolarized to −40 mV from a holding potential of −80 mV compared that of control condition (, *).
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