Flavonoid Myricetin Modulates 𝐆𝐀𝐁𝐀_𝐀 Receptor Activity through Activation of 𝐂𝐚^𝟐+ Channels and CaMK-II Pathway

Figure 4

Myricetin-induced intracellular Ca²⁺ increase in the primary culture of rat hypothalamus neurons and the effects of Ca²⁺ channel blockers. (a) Images of rat hypothalamus neurons before and after 50 μM myricetin perfusion, showing increased Ca²⁺ dye intensity; (b) effect of Ca²⁺ channel blockers on myricetin induced intracellular Ca²⁺ increase (***𝑃<0.001, versus 50 μM myricetin, one-way ANOVA). Mibefradil is T-type Ca²⁺ channel blocker; nifedipine is L-type Ca²⁺ channel blocker. Numbers on the top of each bar represent the numbers of cells examined.

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