7-Hydroxy--Sitosterol from Chisocheton tomentosus Induces Apoptosis via Dysregulation of Cellular Bax/Bcl-2 Ratio and Cell Cycle Arrest by Downregulating ERK1/2 Activation
Figure 3
CT1 potentiates apoptosis-mediated cell death in MCF-7 human breast cancer cells. (a) Detection of apoptosis using flow cytometry after annexin V-FITC/propidium iodide (PI) staining. MCF-7 cells and HMEC cells were treated with CT1 at IC50 concentrations for 24 h. Dot plots are a representative of 1.0 × 104 cells of three replicates with percentage of cells indicated in each quadrant. (b) Confirmation of apoptosis-mediated cell death through observation of a 200 to 250 bp DNA laddering using the DNA fragmentation assay. Cells were treated with CT1 for 24 h followed by analysis of extracted DNA on 1.0% (w/v) agarose gel electrophoresis. +ve: positive control. M: 100 bp DNA size marker.
