7-Hydroxy--Sitosterol from Chisocheton tomentosus Induces Apoptosis via Dysregulation of Cellular Bax/Bcl-2 Ratio and Cell Cycle Arrest by Downregulating ERK1/2 Activation
Figure 7
Activation of caspases upon CT1 treatment in MCF-7 cells. (a) Western blot analysis on protein levels of various procaspases upon CT1 treatment. MCF-7 cells was treated with 16.0 μM of CT1 for 6 h, 12 h, and 24 h, respectively. Western blot of cell extracts were probed using the indicated procaspase antibodies and β-actin as a normalization control. (b) Normalization on band intensities between procaspases and β-actin was determined by densitometry using the ImageJ v1.43 software, and results were presented as a mean normalized intensity ± SEM of three independent experiments.
