7𝜶-Hydroxy-𝜷-Sitosterol from Chisocheton tomentosus Induces Apoptosis via Dysregulation of Cellular Bax/Bcl-2 Ratio and Cell Cycle Arrest by Downregulating ERK1/2 Activation

Table 2

Summary of IC₅₀ values and total cell viability of CT1-treated tumor cell lines and HMEC cells as obtained from MTT cell viability assays after 24 h exposure. All data are presented as mean ± SEM after deduction of DMSO solvent-induced cytotoxicity of three independent experiments.

Cell lines	Time (H)	IC50 (𝜇M)^†	Cell viability (%)
Human mammary epithelial cell (HMEC)	24	n.d	82.4 ± 4.2
	48	n.d	80.3 ± 5.6
Human breast adenocarcinoma (MCF-7)	6	n.d	45.5 ± 5.5
	12	38.2 ± 3.2	36.5 ± 1.9
	18	28.6 ± 4.1	29.3 ± 1.4
	24	16.0 ± 3.6	21.3 ± 3.1
Human hepatocyte carcinoma (HepG2)	6	76.8 ± 5.2	35.9 ± 8.4
	12	74.0 ± 4.3	32.5 ± 9.3
	18	75.0 ± 5.1	26.8 ± 7.2
	24	25.0 ± 3.3	25.2 ± 3.6
Human oral squamous cell carcinoma (HSC-4)	6	30.3 ± 2.4	48.6 ± 1.7
	12	28.8 ± 5.1	48.1 ± 1.0
	18	24.2 ± 3.1	43.2 ± 4.3
	24	19.5 ± 2.6	40.6 ± 3.2
Human cervical carcinoma (Ca Ski)	6	n.d	94.6 ± 4.2
	12	82.9 ± 4.7	73.7 ± 7.7
	18	88.3 ± 6.3	58.8 ± 4.8
	24	96.5 ± 5.3	60.7 ± 3.6

^†n.d denotes that total cell viability was maintained >50% viability at maximum incubation time and CT1 concentration.