Evidence-Based Complementary and Alternative Medicine / 2012 / Article / Fig 4

Research Article

NBM-HD-1: A Novel Histone Deacetylase Inhibitor with Anticancer Activity

Figure 4

Regulation of cell cycle-regulator and tumor suppressor genes in NBM-HD-1-treated cells. (a) C6 cells ( per well) were seeded in 6-well plates and incubated for 14 h then treated with NBM-HD-1 at various concentrations (4.3~21.5 μM) for 48 h. p21(Waf1/Cip1), cyclin B1, and cyclin D1 cell cycle-regulator genes were determined by RT-PCR. (b) Flow cytometric analysis of NBM-HD-1-treated C6 and MCF-7 cells for 48 h and stained with PI. Following the flow cytometric analysis, the cellular DNA profile was analyzed by Cell Quest software. Rat astrocytes ( per well) were seeded in 6-well plates and incubated for 14 h then treated with NBM-HD-1 at various concentrations (4.3–17.0 μM) and 4.0 mM of SB for 48 h. (c) p53 tumor-suppressor genes were determined by RT-PCR. All these tests were performed in three independent experiments. A representative experiment of the three replicates is shown.
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