Evidence-Based Complementary and Alternative Medicine / 2012 / Article / Fig 5

Research Article

NBM-HD-1: A Novel Histone Deacetylase Inhibitor with Anticancer Activity

Figure 5

NBM-HD-1 inhibits cell growth through a PTEN/AKT-dependent pathway. MCF-7 cells ( ) were seeded in 100 mm dishes, incubated for 14 h, and then treated with NBM-HD-1 at a fixed concentration of 17.0 μM for 1, 2, 3, and 4 h. Cell lysates were prepared and subjected to SDS-PAGE and immunoblotting using respective specific antibodies. (a) Ac-histones 4. (b) Ac-tubulin. (c) Phospho-PTEN and phospho-AKT. HDACi biomarkers were determined by a western blot assay.
781417.fig.005

We are committed to sharing findings related to COVID-19 as quickly and safely as possible. Any author submitting a COVID-19 paper should notify us at help@hindawi.com to ensure their research is fast-tracked and made available on a preprint server as soon as possible. We will be providing unlimited waivers of publication charges for accepted articles related to COVID-19. Sign up here as a reviewer to help fast-track new submissions.