Activation of Spinal 𝜶2-Adrenoceptors Using Diluted Bee Venom Stimulation Reduces Cold Allodynia in Neuropathic Pain Rats

Figure 2

Effect of intrathecal adrenoceptor antagonist injection on the DBV-induced antinociception. The effects of intrathecal adrenoceptor antagonists on the DBV-induced antiallodynic effect to cold stimuli in neuropathic pain animals. The saline pretreatment in DBV-treated rats (Sal + DBV) reduced paw withdrawal frequency (PWF, %) to cold stimulus at 60 and 90 minutes compared with the DBV-treated group (Sal + Sal, **𝑃<0.01 and ***𝑃<0.001). The idazoxan-pretreated group (IDX + DBV), but not the PRA- and PRO-treated groups (PRA + DBV and PRO + DBV), significantly reversed PWF from 30 minutes to 90 minutes compared with the saline pretreatment group of DBV-treated rats (Sal + DBV, ^# 𝑃<0.05, ^## 𝑃<0.01 and ^### 𝑃<0.001). All groups were comprised of six animals, respectively. PRA: prazosin, α1-adrenoceptor antagonist, IDX: idazoxzn, α2-adrenoceptor antagonist, PRO: propranolol, nonselective β-adrenoceptor antagonist.