Salvianolic Acid B Reducing Portal Hypertension Depends on Macrophages in Isolated Portal Perfused Rat Livers with Chronic Hepatitis

Figure 5

: Salvianolic acid B reducing portal hypertension in IPPRL with chronic hepatitis. (1) Masson staining was performed to evaluate collagen deposition (100×). (a) Liver with normal structure in normal rats at 𝑑₀. (b) Hepatic degeneration in the portal hypertensive rats at 𝑑₂₈. (c) Hepatic fibrosis in the portal hypertensive rats at 𝑑₅₆. (d) Hepatic cirrhosis in the portal hypertensive rats at 𝑑₈₄. The inserted micrographs in the upper left corner were the portal triad (Masson × 630) from the original ones (black rectangle). (2) Existence of iNOS was detected by immunohistochemistry staining (400×). (e) iNOS was located at the hepatocyte at 𝑑₀. (f) iNOS was located at stellates in the lobules at 𝑑₂₈. (g) iNOS was located at stellates and macrophages at 𝑑₅₆. (h) iNOS was located at macrophages out lobules at 𝑑₈₄. The inserted micrographs in the upper left corner were the portal triad (630×) from the original ones (Black rectangle). (3) Existence of HO-1 was detected by immunohistochemistry staining (400×). (i) HO-1 was located at the hepatocytes only at 𝑑₀. (j) HO-1 was located at the macrophages in portal triads with less at the hepatocytes at 𝑑₂₈ than that at 𝑑₀. (k) HO-1 was located at the macrophages out lobules with lest at the hepatocytes at 𝑑₅₆ among the durations of chronic hepatitis. (l) HO-1 was located at the macrophages in portal triads only at 𝑑₈₄. The inserted micrographs in the upper left corner were the portal triad or its partners (630×) from the original ones (black rectangle). (4) (m) Sal B increased the portal pressure in the IPPRL at 𝑑₀. (n) Sal B decreased the portal pressure in the IPPRL at 𝑑₂₈. (o) Sal B decreased the portal pressure in the IPPRL at 𝑑₅₆. (p) Sal B decreased the portal pressure in the IPPRL at 𝑑₈₄.