The Components of Flemingia macrophylla Attenuate Amyloid β-Protein Accumulation by Regulating Amyloid β-Protein Metabolic Pathway
Figure 7
The effect of the fractions of F. macrophylla on the level of secreted APP in swAPP770-transfected N2a cell. swAPP770-transfected N2a cells were treated with the fractions for 20 h at NTC of 10, 1, and 10 μg/mL, respectively. The level of 6E10 antibody-stained sAPP (i.e., sAPPα) and KPI antibody-stained sAPPs (i.e., sAPPα plus sAPPβ) in conditioned medium was determined by immunoblotting. The upper part is the representative image of immunoblot. The lower part is the relative level of 6E10 antibody-stained sAPPα (opened column) and KPI antibody-stained sAPPs (closed column). Results are means ± SD from three independent experiments. Significant differences between control and FM fractions-treated cells are indicated by *.
