The Components of Flemingia macrophylla Attenuate Amyloid β-Protein Accumulation by Regulating Amyloid β-Protein Metabolic Pathway

Table 1

Extraction and isolation of F. macrophylla. The ground aerial parts of F. macrophylla (12 kg) were extracted following the protocol, and the fractions were named.

Fraction name	The protocol of extraction and fractionation
EtOH	The aerial parts of F. macrophylla were extracted three times with 95% ethanol (EtOH) at 60°C overnight. The combined EtOH extract was evaporated under reduced pressure.
H2O	EtOH extract was taken up in water as water-soluble fraction.
H25M; H50M; H75M; H100M	The water-soluble fraction (H2O) was chromatogramed over Diaion HP-20 column and eluted with 25%-, 50%-, 75%-, and 100%-methanol to give four fractions: H25M, H50M, H75M, and H100M, respectively.
EA; n-BuOH	The water-soluble fraction (H2O) was partitioned with ethyl acetate and n-butanol successively to get two fractions: EA and n-BuOH, respectively.
B25M; B50M; B75M; B100M	The n-BuOH fraction was chromatogramed over Diaion HP-20 column and eluted with 25%-, 50%-, 75%-, and 100%-methanol to give four fractions: B25M, B50M, B75M, B100M, respectively.
EA-n	EA and n-BuOH fractions were subjected to silica gel column chromatography using a hexane-EA-methanol gradient and EA-methanol gradient, respectively. Eleven fractions were collected as EA-n (𝑛=4, 35, 52, 55, 74, 79, 85, 94, 103, 121, 165).
Flavonoids	The fractions rich in flavonoids were separated first over a silica gel column with a 25%–60% EA/hexane gradient as eluent and then over Sephadex LH-20 columns with EA or methanol to afford flavonoids.