Mechanisms of Edible Bird's Nest Extract-Induced Proliferation of Human Adipose-Derived Stem Cells
Figure 2
Cytokine profile analysis of hADSCs treated with EBNE. hADSCs were cultured in the presence of 2,000 ppm EBNE for 60 h. EGF (column 1), FGF-2 (column 2), G-CSF (column 3), IL-6 (column 4), PDGF-BB (column 5), TGF-β1 (column 6), and VEGF (column 7). (a, b) hADSCs were cultured in serum-free DMEM in the presence or absence of 2,000 ppm EBNE, and the concentration of produced cytokines (pg/mL) was measured using a flow cytometric bead assay. Results are shown as mean ± SD in (a) (). * versus serum-free controls. ***NM indicates that treatment with EBNE-induced production of <30 pg/mL of cytokines. Data are representative of at least three independent experiments. (c, d) mRNA levels of IL-6 (c) and VEGF (d) were measured using real-time PCR. Results are shown as mean ± SD (). * versus serum-free controls. Serum (−): serum free, EBNE: 2,000 ppm of EBNE.
