Anti-Inflammatory Effect of Triterpene Saponins Isolated from Blue Cohosh (Caulophyllum thalictroides)
Figure 5
Effects of blue cohosh crude saponin on cytokines mRNA expression in LPS-treated mice. ICR mice were treated by the blue cohosh crude saponin (200 mg/kg) with 1 mg/kg LPS for 24 h. Expression of COX-2, iNOS, TNF-α IL-1β, and IL-6 was measured by PCR analysis. Blue cohosh crude suppressed the COX-2, iNOS, TNF-α IL-1β, and IL-6 in LPS-activated adrenal gland of mice respectively. β actin was used as an internal control. mRNA were collected from adrenal gland of ICR mice after injection of LPS with or without blue cohosh crude extract. All values are expressed as mean ± S.E.M. from three independent experiments. Data were analyzed by one-way ANOVA for multiple comparison and Student-Newman-Keuls test as post hoc test. # in comparison with sham, * in comparison with LPS.
