Terpinen-4-ol Induces Apoptosis in Human Nonsmall Cell Lung Cancer In Vitro and In Vivo
Figure 2
Effects of terpinen-4-ol on cell-cycle distribution in A549 and CL1-0 cells. (a) Cell-cycle analysis of terpinen-4-ol-treated cells. Cells were treated with the indicated concentrations of terpinen-4-ol for 24 hours, and then, cell-cycle distributions were determined by propidium iodide (PI) staining and subsequent flow cytometry analysis (Gating for each cell-cycle phase M1: <2N; M2: G0-G1; M3: S; M4: G2-M). Data are representative of three independent experiments with similar results. (b) Flow cytometry analysis of terpinen-4-ol-induced apoptosis in A549 and CL1-0 cells. The cells were treated with the indicated concentrations of terpinen-4-ol for 24 hours, followed by labeling for phosphatidylserine externalization with FITC-annexin-V and cell membrane integrity with PI. The lower right quadrant (annexin-V+/PI-) represents early apoptosis, while the upper right quadrant (annexin V+/PI+) represents late apoptosis and necrosis. Data are representative of three independent experiments with similar results.
