Inhibition of Activity of GABA Transporter GAT1 by -Opioid Receptor
Effect of opioid receptor coexpression on GAT1-mediated rate of GABA uptake. (a) 40 ng of GAT1 cRNA alone or 40 ng of GAT1 and 20 ng of cRNA for OR were injected into oocytes. Application of 100 nM naloxone gave slight recovery of GAT1 inhibition by the coexpressed OR. (b) 40 ng of GAT1 cRNA alone or 40 ng of GAT1 and 10 ng of cRNA for OR or OR were injected into oocytes. The unspecific rate of uptake was subtracted from the uptake rate of injected oocytes. OR was activated by application of 100 nM DPDPE, OR by 100 nM DAMGO. Data were normalized for each batch of oocytes to the respective value obtained from oocytes not coinjected with cRNA for OR and are presented as means of rates of [3H]GABA uptake SEM from 2 to 4 batches of oocytes (with 5–10 oocytes per batch), .