Inhibition of Activity of GABA Transporter GAT1 by -Opioid Receptor
Effect of OR activation on GAT1-mediated current in rat brain slices from PAG area. Whole-cell patch-clamp recordings were performed on PAG neurons superfusing with different solutions. (a) Steady-state current voltage dependencies of a single experiment with solution sequence as given in the text. The inset shows the GAT1-mediated current determined as tiagabine-sensitive steady-state current. Open squares represent current in the absence and filled squares in the presence of 100 nM DPDPE. (b) GAT1-mediated current from 5 experiments. Data were normalized to the current at −100 mV in the absence of DPDPE and represent averages (SEM). The value of “one” corresponds to pA.