Antimigratory Effects of the Methanol Extract from Momordica charantia on Human Lung Adenocarcinoma CL1 Cells
Figure 3
Effects of MCME on the invasion of CL1-0 and CL1-5 cells. (a) Invasiveness of CL1 cells treated with MCME. MCME induced a dose-dependent inhibition of invasion in CL1 cells. CL1-0 and CL1-5 cells were pretreated with MCME or DMSO (vehicle control) for 24 h and then seeded onto the transwell chamber. Photographs were taken with an inverted microscope with 10x magnification. (b) The invasive abilities of CL1 cells were quantified by enumerating the stained cells that invaded into the underside of the porous polycarbonate membrane. (c) Expression and phosphorylation of Src and FAK in CL1-0 and CL1-5 cells after treatment with MCME at 0 ~ 1.25 mg/mL for 24 h. (d) The protein activities were normalized to β-actin and expressed as the fold change to the respective control (0 h). Data derived from three independent experiments were represented by mean ± SEM. * and ** indicate and 0.01, respectively. # indicates the difference between CL1-0 and CL1-5 cells is significant ().
