Topical Application of Chrysanthemum indicum L. Attenuates the Development of Atopic Dermatitis-Like Skin Lesions by Suppressing Serum IgE Levels, IFN-γ, and IL-4 in Nc/Nga Mice
Figure 2
Serum levels of IgG1, IgE, IL-4, and IFN-γ levels at the end of experimental periods. AD was induced in Nc/Nga mice by topical application of 2,4-dinitrochlorobenzene (DNCB) in the dorsal skin, and a right ear was topically treated with 1,3-butylen glycol (BG; control), 5% Chrysanthemum indicum L. (CIL-Low), 30% Chrysanthemum indicum L. (CIL-High), and 0.1% hydrocortisone (HC) on the lesions for 5 weeks. Normal controls did not have DCNB-induced AD. After 5 weeks of treatments, serum was separated to measure immunoglobulins and cytokines. CIL-High significantly reduced circulating levels of cytokines and immunoglobulins in comparison to the control, and CIL-High exhibited a statistically similar amelioration to HC. (a) Serum levels of IgG1 and IgE. (b) Serum levels of IL-4 and INF-γ. Each value represents the mean ± SD of 10 mice in each group. *Significantly different among the different treatments in Nc/Nga mice at . a, b, c Means on the bars with different superscripts were significantly different among Nc/Nga mice by Tukey test at . †Significantly different between control (BG) and normal control at .
