Identification of Major Active Ingredients Responsible for Burn Wound Healing of Centella asiatica Herbs
Figure 2
Effects of asiaticoside (AD), madecassoside (MD), asiatic acid (AA), and madecassic acid (MA) on the proliferation of huamn skin fibroblasts. (a) Representative pictures of immunofluorescence staining for EdU (red) and Hoechst 33342 (blue) in the nucleus of cells after treated with the four compounds for 48 h (magnification 250x). (b) Proliferation rates were calculated by normalizing the number of EdU-positive cells to the Hoechst-stained cells in nine fields at 250x magnification. Each column represents the mean ± S.D. from three independent experiments.
