Identification of Major Active Ingredients Responsible for Burn Wound Healing of Centella asiatica Herbs

Figure 4

Effects of asiaticoside (AD), madecassoside (MD), asiatic acid (AA), and madecassic acid (MA) on the expressions of MMP-1 and TIMP-1 mRNA in human skin fibroblasts. The cells were treated with various concentrations of the four triterpene compounds for 24 h. ((a)–(c)). The mRNA expressions of MMP-1 and TIMP-1 were measured via RT-PCR assay. (d) The ratios of relative intensity between MMP-1 mRNA and TIMP-1 mRNA were calculated. Each column represents the mean ± S.D. from three independent experiments. *𝑃<0.05 versus control.

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