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Evidence-Based Complementary and Alternative Medicine
Volume 2012, Article ID 848269, 6 pages
Research Article

Mechanism by Sambucus nigra Extract Improves Bone Mineral Density in Experimental Diabetes

1Department of Cellular and Molecular Biology, University of Medicine and Pharmacy “Grigore T. Popa” Iasi, 16 Universitatii Street, 700115 Iasi, Romania
2Department of Pathophysiology, University of Medicine and Pharmacy “Grigore T. Popa” Iasi, 16 Universitatii Street, 700115 Iasi, Romania

Received 31 May 2012; Revised 10 August 2012; Accepted 22 August 2012

Academic Editor: HuanBiao Mo

Copyright © 2012 Laurentiu Badescu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The effects of polyphenols extracted from Sambucus nigra fruit were studied in streptozotocin- (STZ-) induced hyperglycemic rats to evaluate its possible antioxidant, anti-inflammatory, antiglycosylation activity, and antiosteoporosis effects in diabetes. DEXA bone mineral density tests were performed in order to determine bone mineral density (BMD), bone mineral content (BMC), and fat (%Fat) in control and diabetic animals, before and after polyphenol delivery. As compared to the normoglycemic group, the rats treated with STZ (60 mg/kg body weight) revealed a significant malondialdehyde (MDA) increase, as an index of the lipid peroxidation level, by 69%, while the total antioxidant activity (TAS) dropped by 36%, with a consistently significant decrease ( 𝑃 < 0 . 0 5 ) in the activity of superoxide dismutase (SOD) and glutathione peroxidase (GPX). Also, the treatment of rats with STZ revealed a significant increase of IL-6, glycosylated haemoglobin (HbA1c), and osteopenia detected by DEXA bone mineral density tests. The recorded results highlight a significant improvement ( 𝑃 < 0 . 0 0 1 ) in the antioxidative capacity of the serum in diabetic rats treated with natural polyphenols, bringing back to normal the concentration of reduced glutathione (GSH), as well as an important decrease in the serum concentration of MDA, with improved osteoporosis status. Knowing the effects of polyphenols could lead to the use of the polyphenolic extract of Sambucus nigra as a dietary supplement in diabetic osteoporosis.