Bioguided Fractionation Shows Cassia alata Extract to Inhibit Staphylococcus epidermidis and Pseudomonas aeruginosa Growth and Biofilm Formation

Table 2

High-resolution mass spectrometric data of major secondary metabolites identified in the HPLC fractions by qTOF-MS/MS.

Chemical class	Fraction	Molecular weight	MS^1 (m/z)	MS^2 (m/z)^a	Identified compound
Flavonoids	F6	594.15847	593.1835 [M-H]^−	593.1453, 429.0766, 284.0342, 255.0322	Kaempferol-O-diglucoside
	F6	464.095476	463.1127 [M-H]^-	463.0873, 301.0361, 300.0290, 255.0326, 179.0036	Quercetin-O-glucoside
	F11	448.100561	447.1187 [M-H]^-	447.1440, 284.0699, 255.0648, 227.0681, 151.0299	Kaempferol-O-glucoside
	F18	286.047738	285.0687 [M-H]^-	285.0738, 229.0806, 185.0870, 171.0709	Kaempferol
Anthraquinones	F20	284.032088	283.0597 [M-H]^-	283.2830, 257.0650, 239.0532, 211.0573, 183.0626	Rhein
	F20	240.042259	239.0664 [M-H]^-	239.0538, 211.0580, 183.0630	Danthron

^aIons in bold face indicate the most intense product ion (100% relative intensity). Compounds present in F13 were not identified.