HRT, Herbal Formula, Induces G2/M Cell Cycle Arrest and Apoptosis via Suppressing Akt Signaling Pathway in Human Colon Cancer Cells
Figure 2
Cytotoxic effects of HRT and its constituents. (a) Stomach (AGS), epidermoid (A431), lung (A549), kidney (Caki-1), colon (HCT116), cervical (HeLa), prostate (PC-3), and liver (SK-Hep-1) cancer cells were treated with HRT (300 μg/mL), incubated for 48 h and examined for antitumor effect. (b) Water extract of HRT and four constituent herbs in HRT were treated at concentration of 75 μg/mL and 300 μg/mL on HCT116 cells for 48 h, respectively. HRT significantly inhibited the proliferation of HCT116 cells and showed synergistic anticancer effect, compared with constituent herbs. Pa, P. amurense; Cj, C. japonica; Sb, S. baicalensis; Gj, G. jasminoides. (c) After treatment with HRT or four constituent herbs in HRT at concentration of 75 μg/mL and 300 μg/mL on HCT116 cells, the amount of LDH released into medium were measured using ELISA assay. (d) HCT116 cells were treated with HRT (300 μg/mL) and incubated for 24 h and 48 h, respectively. The antiproliferative effects induced by HRT on HCT116 cells were increased with the incubation times, and results of LDH assay identified HRT induces apoptosis not necrosis effect in colon cancer cells. The data with present mean ± S.D. , and versus untreated cells.
