Synergistic Apoptosis-Inducing Antileukemic Effects of Arsenic Trioxide and Mucuna macrocarpa Stem Extract in Human Leukemic Cells via a Reactive Oxygen Species-Dependent Mechanism
Figure 1
Antiproliferative effects of combined application of arsenic trioxide (ATO) and crude methanolic extract of Mucuna macrocarpa (CMEMM) on human leukemia cells. HL-60, Jurkat, or Molt-3 cells (1 × 105 cells/mL) were seeded into 6-well plates and exposed to 0, 2.5, or 5 μM ATO alone or together with 0, 25, 50, or 75 μg/mL CMEMM for 24 and 48 h. Vehicle control cells were treated with 0.1% DMSO in medium. The percentages of cell growth were measured by the trypan blue exclusion assay and calculated by comparing the cells number with that of the vehicle controls. Each value represents the mean ± S.E. of duplicate cultures from three independent experiments. *, ** indicate significant difference from the respective control value.
