Synergistic Apoptosis-Inducing Antileukemic Effects of Arsenic Trioxide and Mucuna macrocarpa Stem Extract in Human Leukemic Cells via a Reactive Oxygen Species-Dependent Mechanism
Figure 2
Cell cycle progression in leukemia cells exposed to arsenic trioxide (ATO) and/or crude methanolic extract of Mucuna macrocarpa (CMEMM). HL-60 (a) or Jurkat (b) cells (1 × 105 cells/mL) were first treated with 5 mM N-acetyl cysteine (NAC) or untreated, followed by treatment with 0.1% DMSO (CTL), 2.5 μM ATO, and/or 50 μg/mL CMEMM as indicated. After 24 or 48 h of treatment, cells were collected and stained with propidium iodide and determined for DNA content using flow cytometry. The percentages of sub-G1 or hypodiploid cells were analyzed by ModFit LT software. The representative cell cycle progressions in ATO- and/or CMEMM-treated or control cells were from one of three independent experiments.
