Synergistic Apoptosis-Inducing Antileukemic Effects of Arsenic Trioxide and Mucuna macrocarpa Stem Extract in Human Leukemic Cells via a Reactive Oxygen Species-Dependent Mechanism
Figure 3
Nuclear morphological changes induced by arsenic trioxide (ATO) and/or crude methanolic extract of Mucuna macrocarpa (CMEMM). HL-60, Jurkat, or Molt-3 cells (1 × 105 cells/mL) were treated with 0.1% DMSO (control), 2.5 μM ATO, 50 μg/mL CMEMM, or 2.5 μM ATO plus 50 μg/mL CMEMM. After 24 or 48 h of incubation, cells were washed with PBS and collected on microscope slides by cytospin. The nuclei were stained with 2.5 μg/mL DAPI. Arrows indicate apoptotic bodies of nuclear fragmentation. Magnification × 200.
