Synergistic Apoptosis-Inducing Antileukemic Effects of Arsenic Trioxide and Mucuna macrocarpa Stem Extract in Human Leukemic Cells via a Reactive Oxygen Species-Dependent Mechanism
Figure 5
Annexin V-FITC/propidium iodide (PI) analyses of arsenic trioxide (ATO)- and/or crude methanolic extract of Mucuna macrocarpa (CMEMM)-treated cells. HL-60 (a) and Jurkat (b) cells (1 × 105 cells/mL) were first treated with 5 mM N-acetyl cysteine (NAC), 50 μM butylated hydroxytoluene (BHT), or 40 μM α-tocopherol (VitE) or untreated, followed by treatment with 0.1% DMSO (CTL), 2.5 μM ATO, and/or 50 μg/mL CMEMM as indicated for 24 h. Quantitative percentages of apoptotic cells of ATO/CMEMM-treated cells were measured by flow cytometry. Data represent the result from one of three independent experiments.
