Phyllanthus urinaria Induces Apoptosis in Human Osteosarcoma 143B Cells via Activation of Fas/FasL- and Mitochondria-Mediated Pathways
Figure 4
P. urinaria enhanced the Fas and FasL expression in 143B cells, but to a lesser extent in 143Bρ 0 cells. Cell lysates treated with P. urinaria for 24 hours (a) and 48 hour (b) were subjected to Western blot analysis. Cells were pretreated with P. urinaria for 24 (c, e) and 48 (d, f) hours and incubated with Fas or FasL antibodies one hour before harvest and then stained for Fas or FasL with anti-IgG3 Alexa 546- or 488-conjugated secondary antibodies (red or green). DAPI staining was used to detect all nuclei and visualized under a fluorescence microscope (1000x). Flow cytometry analysis of surface FasL. Quantitation of surface FasL levels in 143 B and 143Bρ 0 cells treated with P. urinaria for 24 (g) and 48 (h) hours after Alexa 488-conjugated secondary antibodies was determined by flow cytometry analysis. An asterisk represents statistical significance from the vehicle control (**). A pound sign represents statistical significance between 143B and 143Bρ 0 cells (#).
