Research Article

Antiviral Activity of Isatis indigotica Extract and Its Derived Indirubin against Japanese Encephalitis Virus

Figure 3

Inhibition of virus attachment by I. indigotica extract, indigo and indirubin. I. indigotica extracts (a) indigo and indirubin (b) were serially diluted and mixed with JEV (120 pfu), each mixture incubated 1 h with BHK-21 cell monolayers at 4°C. Then virus/compound mixture was removed and cell monolayer washed with cold PBS. Residual infectivity was performed as described in plaque assay.
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(a)
925830.fig.003b
(b)