Evidence-Based Complementary and Alternative Medicine / 2012 / Article / Fig 4

Research Article

Antiviral Activity of Isatis indigotica Extract and Its Derived Indirubin against Japanese Encephalitis Virus

Figure 4

Virucidal activities of I. indigotica and its major components. I. indigotica extracts (a) indigo and indirubin (b) were serially diluted and mixed with JEV. Each virus/compound mixture was incubated at 4°C for 1 h, then added onto the BHK-21 cell monolayer at 37°C for another 1 h. The virus/compound mixture was removed from 6-well plates and cell monolayer washed with PBS. Residual infectivity was performed as described in plaque assay.

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