Antiviral Activity of Isatis indigotica Extract and Its Derived Indirubin against Japanese Encephalitis Virus

Table 2

Antiviral effect of I. indigotica extracts and its marker components against JEV.

Isatis  indigotica	IC50 (𝜇g/mL) of pretreatment	IC50 (𝜇g/mL) of simultaneous treatment	IC50 (𝜇g/mL) of posttreatment	IC50 (𝜇g/mL) of virus attachment	IC50 (𝜇g/mL) of virucidal assay
Ethyl acetate extract	138.81±3.71	91.57±3.54	>200	>200	65.79±3.54
Methanol extract	85.46±8.54	78.47±3.06	>200	50.57±2.12	22.17±3.06
Indigo	11.79±0.10	37.49±3.18	>50	5.15±0.18	3.03±3.18
Indirubin	23.50±0.89	13.68±2.83	>50	5.10±1.32	0.47±2.83