Tanshinone IIA Inhibits Growth of Keratinocytes through Cell Cycle Arrest and Apoptosis: Underlying Treatment Mechanism of Psoriasis
Figure 11
Effect of tanshinone IIA on cell cycle distribution in keratinocytes. (a) Tanshinone IIA treatment resulted in a time-dependent increase in the proportion of cells in the S and G2/M phases and a decrease in the proportion of cells in the G1 phase. (b) Histograms represent means ± SD of the percent of cell cycle distributions for different concentrations of tanshinone IIA (2.5–10 μg/mL). The asterisk indicates a significant difference between control and tanshinone IIA-treated cells (*). (c) Expression of cyclin A and pCDK2, which are related to S phase of the cell cycle, was found to be decreased, and expression of PCNA, which is related to cell proliferation, was also downregulated compared to the control. Expression of tubulin was used as a protein loading control.
