Tanshinone IIA Inhibits Growth of Keratinocytes through Cell Cycle Arrest and Apoptosis: Underlying Treatment Mechanism of Psoriasis

Figure 9

Tanshinone IIA-induced apoptosis is mediated without AIF translocation. (a) Keratinocytes were treated with tanshinone IIA (5 and 10 μg/mL) for 24 h, incubated with antibody against AIF, and Alexa Fluor 488- or 594-conjugated secondary antibodies (Invitrogen) were used for immunofluorescence staining. Nuclei were secondarily stained with DAPI. Purple nuclei due to overlap of AIF (red fluorescence) and nuclear staining (blue fluorescence) means translocation of AIF was not observed. The scale bar in the first panel represents 50 μm for both sections. (b) Western blot analysis showed no difference in AIF expression between the nucleus and cytoplasm.