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Evidence-Based Complementary and Alternative Medicine
Volume 2012, Article ID 952504, 8 pages
Research Article

Rhein Induces Apoptosis in Human Breast Cancer Cells

1Department of Biotechnology, Asia University, Taichung 413, Taiwan
2Institute of Bioinformatics and Structural Biology, Department of Medical Science, National Tsing Hua University, Hsinchu 300, Taiwan
3School of Pharmacy, China Medical University, Taichung 404, Taiwan
4Department of Biological Science and Technology, China Medical University, Taichung 404, Taiwan
5Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung 404, Taiwan
6Department of Medical Genetics and Medical Research, China Medical University Hospital, Taichung 404, Taiwan

Received 6 May 2011; Revised 19 July 2011; Accepted 12 August 2011

Academic Editor: Richard Pietras

Copyright © 2012 Ching-Yao Chang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplemental Figure 1: To compare cytotoxic effect of rhein on normal breast MCF-10A cells, and breast cancer MCF-7/VEC and MCF-7/HER2 cells, the cells were treated with 20 μg/mL rhein for 48 h. The cell survival rate was determined using MTT assay, showing 99.7 ± 0.4% for MCF-10A cells, 75.5 ± 3.5% for MCF-7/VEC cells, and 59.1 ± 1.4% for MCF-7/HER2 cells, respectively. The resulted demonstrated rhein being less toxic to non-tumorigenic MCF-10A cells.

  1. Supplementary Material