Pseudolaric Acid B Induces Caspase-Dependent and Caspase-Independent Apoptosis in U87 Glioblastoma Cells
Figure 3
Nuclear morphological changes of U87 glioblastoma cells. (a) U87 cells were treated with DMSO or 5 and 10 μM PLAB for 24 h. After fixing with 4% paraformaldehyde at room temperature for 30 min, cells were stained with Hoechst 33258 (50 g/mL) in the dark for 30 min. After washing, cells were resuspended in PBS solution and nuclear morphological changes were observed by fluorescence microscope. Finally, 100 nuclei were counted microscopically from each group for the percentage of cleaved nuclei (Apoptosis). (b) Data are expressed as Mean ± SEM (). Columns not sharing the same superscript letter differ significantly ().
