Hochuekkito (TJ-41), a Kampo Formula, Ameliorates Cachexia Induced by Colon 26 Adenocarcinoma in Mice
Figure 4
Effect of hochuekkito on macrophage infiltration and IL-6 production by macrophages or cancer cells from C26-bearing mice. (a) Immunostaining of IL-6 in tumors from C26-bearing mice that had been treated with or without hochuekkito, 15 days after inoculation of cancer cells. Scale bars: 100 μm. (b) Immunostaining of Mac1/CD11b and IL-6 in tissues surrounding tumors from C26-bearing mice with or without treatment with hochuekkito, 15 days after inoculation of cancer cells. Serial sections from the same tissue sample were used for analysis. Scale bars: 100 μm. (c) Quantitative immunohistochemical analysis of macrophage infiltration. The bar graph shows the fold-change in macrophage infiltration in tissues surrounding tumors in C26-bearing mice, treated with or without hochuekkito, calculated by setting the ratios of macrophage counts in untreated mice as 1. *Statistically significant difference compared with the group of hochuekkito (−); (d) Quantitative immunohistochemical analysis for IL-6 production by macrophages in tissues surrounding tumors in C26-bearing mice treated with or without hochuekkito. The area positive for Mac-1 or IL-6 immunoreactivity was quantified in 4 fields per slide. IL-6 production by macrophages was estimated by determining the ratio of the IL-6-positive area to the Mac-1-positive area. Quantitative data are presented as means ± SD. Statistical significance was evaluated with unpaired Student’s -test. *Statistically significant difference compared with the group of hochuekkito (−); .
