Diosgenin Induces Apoptosis in HepG2 Cells through Generation of Reactive Oxygen Species and Mitochondrial Pathway
Figure 1
Cytotoxic Effect of diosgenin in HepG2 cells. (a) Cells were treated with diosgenin by dose-dependent manner for 24 and 48 h. The ratios of cell viability were measured by MTT assay. Data are presented as mean ± SD of six replicates from three independent experiments. ** compared to control. (b) Nuclear alterations were observed by DAPI staining and fluorescence microscopy (×100). After cells were treated with diosgenin (0–40 μM) for 48 h, marked morphological changes of cell apoptosis such as condensation of chromatin and nuclear fragmentations were found clearly using DAPI staining.
