Diosgenin Induces Apoptosis in HepG2 Cells through Generation of Reactive Oxygen Species and Mitochondrial Pathway
Figure 2
FACS analyses of Annexin V and PI staining. HepG2 cells was treated with diosgenin (0–40 μM) for 24 h (a) and 40 μM for 0, 24, 48 h (b). Lower right quadrant, early apoptosis cells, that is, Annexin V-FITC-positive/PI-negative cells; upper right quadrant, necrosis or late-apoptotic cells, that is, Annexin V-FITC-positive/PI-positive cells.
