Diosgenin Induces Apoptosis in HepG2 Cells through Generation of Reactive Oxygen Species and Mitochondrial Pathway
Figure 4
Diosgenin generated ROS in HepG2 cells. (a) Diosgenin generated ROS in HepG2 cells. Cells were treated with different concentrations of diosgenin (20–40 μM) plus 40 μM of DCFH-DA for 24 h, and ROS productions were determined by FACS analysis. (b) For observation of intracellular ROS by fluorescence microscope, cells were treated with diosgenin (20–40 μM) and then incubated with DCFH-DA.
