Diosgenin Induces Apoptosis in HepG2 Cells through Generation of Reactive Oxygen Species and Mitochondrial Pathway

Figure 5

Diosgenin-generated ROS induced apoptosis in HepG2 cells. (a) The cells were pretreated with/without NAC (10 mM) at least 2 hr before the treatment of 40 μM diosgenin. After 24 h, quantitative assessment of the percentage of cell viability was determined by MTT assay. **𝑃<0.01 compared to control, ^##𝑃<0.01 compared to diosgenin 40 μM-treated group. (b) Also cells were pretreated with/without NAC (10 mM) at least 2 h before the treatment of diosgenin. ROS production was confirmed by fluorescence microscope.

(a)

(b)