Selected Extracts of Chinese Herbal Medicines: Their Effect on NF-κB, PPARα and PPARγ and the Respective Bioactive Compounds
Figure 2
Comparison of the PPARα- and PPARγ-activating potential of the VLC fractions petroleum ether (PE), DCM-I and -II, and ethyl acetate extract (EtOAc) of the DCM extract from A. julibrissin no. 1a (a) and the DCM fractions D1-8 from Arisaema sp. no. 2a. (b). HEK293 cells, transiently transfected with expression plasmids for PPARα or PPARγ, luciferase reporter (tk-PPREx3-luc), and EGFP as internal control, were incubated for 18 h with 10 μg/mL of the indicated fraction, solvent vehicle (0.1% DMSO, negative control), and 50 nM GW7647 (PPARα agonist) or 5 μM troglitazone (PPARγ agonist) as positive control. Luciferase activity and fluorescence intensity were measured. Results are presented as mean ± SD (). Significantly different from the negative control (ANOVA), *; **.
