Evidence-Based Complementary and Alternative Medicine / 2013 / Article / Tab 1

Research Article

Evaluation of Acute 13-Week Subchronic Toxicity and Genotoxicity of the Powdered Root of Tongkat Ali (Eurycoma longifolia Jack)

Table 1

Mutagenicity assay of E. longifolia powdered root in vitro (Ames test).

TA98TA100TA102TA1535TA1537

Without S9 metabolic activation
Negative1
Positive2
E. longifolia powdered root (mg/plate)
 0.3125
 0.625
 1.25
 2.5
 5

With S9 metabolic activation
Negative1
Positive2
E. longifolia powdered root (mg/plate)
 0.3125
 0.625
 1.25
 2.5
 5

The values were presented as Mean ± S.D. . No significant changes of revertants were observed at any concentrations of test compound treatments in five tester strains. Statistical analysis: indicates a statistical difference with the control group by one-way ANOVA when the number of revertants was twice than control at TA98, TA100, and TA102 and triple than control at TA1537 and TA1535.
Solvent (sterile water) was used as negative control.
Positive control in w/o S9 plate: TA98 (4-nitro-o-phenylenediamine 2.5 μg/plate); TA100 and TA1535 (sodium azide 5 μg/plate); TA102 (mitomycin C 0.5 μg/plate); TA1537 (9-aminoacridine 50 μg/plate); in w/w S9 plate: TA100/TA102/TA1535/TA1537 (2-aminoanthrene 5 μg/plate); TA98 (benzo(a)pyrene 5 μg/plate).

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